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Literature summary extracted from

  • Li, W.T.; Mahapatra, A.; Longstaff, D.G.; Bechtel, J.; Zhao, G.; Kang, P.T.; Chan, M.K.; Krzycki, J.A.
    Specificity of pyrrolysyl-tRNA synthetase for pyrrolysine and pyrrolysine analogs. (2009), J. Mol. Biol., 385, 1156-1164.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.26 0.055
-
 L-pyrrolysine pH 7.2, 37°C Methanosarcina barkeri
6.1.1.26 0.39
-
 2-amino-6-((R)-tetrahydrofuran-2-carboxamido)hexanoic acid pH 7.2, 37°C Methanosarcina barkeri
6.1.1.26 0.55
-
 Nepsilon-cyclopentyloxycarbonyl-L-lysine pH 7.2, 37°C Methanosarcina barkeri
6.1.1.26 5.6
-
 2-amino-6-(cyclopentanecarboxamido)hexanoic acid pH 7.2, 37°C Methanosarcina barkeri

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.26 Methanosarcina barkeri
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.26 ATP + 2-amino-6-((R)-tetrahydrofuran-2-carboxamido)hexanoic acid + tRNAPyl catalytic efficiency (kcat/Km) is 9% of the catalytic efficiency for L-pyrrolysine Methanosarcina barkeri AMP + diphosphate + 2-amino-6-((R)-tetrahydrofuran-2-carboxamido)hexanoyl-tRNAPyl
-
 ?
6.1.1.26 ATP + 2-amino-6-(cyclopentanecarboxamido)hexanoic acid + tRNAPyl catalytic efficiency (kcat/Km) is 0.3% of the catalytic efficiency for L-pyrrolysine Methanosarcina barkeri AMP + diphosphate + 2-amino-6-(cyclopentanecarboxamido)hexanoyl-tRNAPyl
-
 ?
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl
-
 Methanosarcina barkeri AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 ATP + Nepsilon-cyclopentyloxycarbonyl-L-lysine + tRNAPyl catalytic efficiency (kcat/Km) is 1.8% of the catalytic efficiency for L-pyrrolysine Methanosarcina barkeri AMP + diphosphate + Nepsilon-cyclopentyloxycarbonyl-L-lysyl-tRNAPyl
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.26 PylS
-
 Methanosarcina barkeri
6.1.1.26 pyrrolysyl-tRNA synthetase
-
 Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.26 37
-
 assay at Methanosarcina barkeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.26 0.015
-
 Nepsilon-cyclopentyloxycarbonyl-L-lysine pH 7.2, 37°C Methanosarcina barkeri
6.1.1.26 0.04
-
 2-amino-6-(cyclopentanecarboxamido)hexanoic acid pH 7.2, 37°C Methanosarcina barkeri
6.1.1.26 0.062
-
 2-amino-6-((R)-tetrahydrofuran-2-carboxamido)hexanoic acid pH 7.2, 37°C Methanosarcina barkeri
6.1.1.26 0.105
-
 L-pyrrolysine pH 7.2, 37°C Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.26 7.2
-
 assay at Methanosarcina barkeri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.26 0.0071
-
 2-amino-6-(cyclopentanecarboxamido)hexanoic acid pH 7.2, 37°C Methanosarcina barkeri
6.1.1.26 0.027
-
 Nepsilon-cyclopentyloxycarbonyl-L-lysine pH 7.2, 37°C Methanosarcina barkeri
6.1.1.26 0.16
-
 2-amino-6-((R)-tetrahydrofuran-2-carboxamido)hexanoic acid pH 7.2, 37°C Methanosarcina barkeri
6.1.1.26 1.9
-
 L-pyrrolysine pH 7.2, 37°C Methanosarcina barkeri